FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_input_180_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_input_180_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12784179
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14150.022.01283846
ATGCCGT15950.019.3092147
TGCCGTC16450.019.1478148
CGTATGC16150.019.07031244
GCCGTCT17300.018.00479349
GTATGCC18050.017.25680545
GCGGCCT32250.011.8306611
AACGCGG33150.011.2984598
GCTGGCC37000.010.68881139
CGGCCTT37000.010.406312
CCTGCGT36850.010.06764565
TCCTGGC38900.09.98671527
CGCGGCC39250.09.81010510
CCGTCTT34250.09.50320250
GCGTTAT39900.09.47368368
AGTCACC35300.09.22078128
TGCGTTA43750.09.03992867
CTCGTAT38900.08.90707142
TCTCGTA34800.08.85026241
TCGTATG39600.08.661242543
Produced by FastQC (version 0.11.2)