FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_input_10_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_input_10_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10565600
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11600.017.80052846
GCCGTCT12150.016.41872849
ATGCCGT12450.016.0230247
TGCCGTC12700.015.43210748
CGTATGC12600.014.99913344
GTATGCC13700.014.81666345
GCGGCCT30800.012.27381911
AACGCGG31200.012.2294058
CCTGCGT32900.011.48902465
CGGCCTT34000.011.32437612
CGCGGCC36850.011.20896710
GCTGGCC33650.011.12862939
TCCTGGC35000.011.09962227
GCGTTAT34850.010.54508468
TGCGTTA38900.09.71712367
CCTGGCC42450.09.64654328
ACGCGGC42300.09.3514229
TCCTGCG42150.08.88463264
TTACGGT46600.08.78733720
AGCAACG47750.08.65047555
Produced by FastQC (version 0.11.2)