FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_input_0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_input_0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283642
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20600.033.46914746
GCCGTCT21800.032.2689849
ATGCCGT21450.032.14274247
GTATGCC22200.031.05730645
TGCCGTC22750.030.76752748
CGTATGC23250.029.95578244
CCGTCTT39950.018.22190750
TCTCGTA39350.018.0551841
CATCTCG38800.018.04044239
ATCTCGT41250.016.88416740
AGTCACC43200.016.52742228
CTCGTAT43600.016.1346742
TCGTATG44800.016.01497843
ACGTCTG52300.013.45085115
CCAGATC53700.013.16555233
CTGCTTG56400.012.59691557
CGTCTGA56550.012.44005116
ACCAGAT57100.012.38161332
ACTCCAG59300.011.980741523
GCACACG58300.011.82788411
Produced by FastQC (version 0.11.2)