FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_60_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_60_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7674996
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC174260.2270489782665685TruSeq Adapter, Index 5 (100% over 50bp)
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG118270.15409780018126393No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86440.11262546586343497No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT85000.11074924338722783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28000.047.8734144
TATGCCG28150.047.74170746
GTATGCC29300.046.5854845
ATGCCGT28850.046.3407147
GCCGTCT29150.045.74402649
TGCCGTC29500.045.3196448
TCTCGTA35950.037.77367841
CCGTCTT37750.035.5082950
CTCGTAT42300.032.6821342
ATCTCGT41950.031.78699140
CACAGTG42650.031.2656933
TCGTATG46050.029.79271343
CAGTGAT45400.029.4483635
CTGCTTG49800.027.2681757
ACAGTGA49300.027.19029834
AGTGATC50650.027.01785936
GTCACAC52200.025.9479229
TGCTTGA55350.024.59719758
GTGATCT57000.023.76252637
GCTTGAA58050.023.39269459
Produced by FastQC (version 0.11.2)