FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_5_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_5_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11841366
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC427360.36090430783070127TruSeq Adapter, Index 2 (100% over 50bp)
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG413670.3493431416611901No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT252210.21299062962837229No Hit
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC170730.1441810007392728No Hit
CCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAA140560.11870252131384167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT51500.061.90854647
TATGCCG51550.061.84850346
GTATGCC51650.061.59401345
TGCCGTC51950.061.30491648
CGTATGC52250.060.8867144
GCCGTCT52400.060.77869849
CCGTCTT54500.058.62941750
TCTCGTA55800.057.0758241
ATCTCGT56850.056.0216540
GCTTGAA60500.052.81537659
TGCTTGA62050.051.32684758
CCGATGT71750.044.43910233
ACCGATG75500.042.185532
CTGCTTG84150.038.63728357
TATCTCG84200.038.48964339
TCACCGA84850.037.49485430
TTCTGCT87400.036.8401555
GTCACCG88200.036.03043729
CGATGTA88850.035.9651734
TCTGCTT92750.034.75257556