FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_30_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_30_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9175079
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC394820.4303178206966937TruSeq Adapter, Index 4 (100% over 50bp)
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG147520.16078335674275937No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT106930.1165439556433247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50000.058.79754346
GCCGTCT52200.056.31948549
ATGCCGT52500.055.99735347
TGCCGTC52750.055.86496448
CGTATGC53100.055.6950844
GTATGCC53650.055.05887645
TCTCGTA61550.047.8785641
CCGTCTT62400.047.39436750
CTCGTAT69200.043.19230742
ATCTCGT68500.043.17408440
TCGTATG72450.041.68953343
CTGCTTG73200.040.8794457
AATCTCG78600.037.7598539
ACTGACC81150.036.401232
CTGACCA83800.035.2500833
TGCTTGA86000.034.7136758
ACCAATC86600.034.35322636
GCTTGAA88400.033.6524459
CCAATCT90900.032.61264437
GACCAAT96150.030.97718635
Produced by FastQC (version 0.11.2)