FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_300_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_300_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8234144
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT487520.5920712584088886TruSeq Adapter, Index 14 (97% over 44bp)
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG242850.29493047486174645No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT172550.20955426575002817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87050.1057183357492898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58250.061.1646548
GTATGCC58350.061.0590947
TGCCGTC58800.060.53301650
CGTATGC59300.060.19894446
GCCGTCT59200.060.12400451
ATGCCGT59450.059.9300449
TCTCGTA62900.056.42003343
CCGTCTT64850.055.0476652
CGTATCT66150.053.3306339
ATCTCGT66950.053.05929642
GTTCCGT68600.051.42752535
TTCCGTA70200.050.40495736
TGCTTGA75000.047.7848360
AGTTCCG74800.047.21246734
CCGTATC75100.046.97498338
GCTTGAA76850.046.6800561
TCCGTAT77900.045.6015837
CTGCTTG80700.044.23621459
TATCTCG80550.044.0139141
CACAGTT82200.043.1750931
Produced by FastQC (version 0.11.2)