FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8316784
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT650530.7821893655047432TruSeq Adapter, Index 13 (97% over 40bp)
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG266600.32055659976260054No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT202350.24330318065252146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT74750.061.05451649
TATGCCG75300.060.56208848
CGTATGC75650.060.42032646
GCCGTCT75900.060.08261551
GTATGCC76250.059.85308547
TGCCGTC76350.059.82089250
TCTCGTA80300.056.74649443
CCGTCTT82900.055.09372752
ATCTCGT84050.054.17303542
GCTTGAA94000.048.7369161
TGCTTGA94550.048.52743560
CTGCTTG96750.047.27898859
ACAGTCA98350.046.4773232
ACAATCT99000.046.3105239
CTCGTAT98600.046.2859844
TCGTATG100350.045.5834345
AATCTCG100650.045.44701841
TTCTGCT106250.043.9410557
AGTCAAC111300.041.44694534
TCTGCTT111250.041.27408258
Produced by FastQC (version 0.11.2)