FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_180_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_180_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8921116
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG291770.32705549395389544No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT178900.20053544870395137No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC125640.140834397848879TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18500.048.05183446
ATGCCGT18950.047.0954547
TGCCGTC19050.046.66477648
GCCGTCT19100.046.3593849
GTATGCC19550.045.65032645
CGTATGC20400.043.91979244
CCGTCTT25200.035.41523450
TCTCGTA25150.035.3464841
ATCTCGT28300.031.41215340
TGGGGGG6550.030.4805281
CACGCCA31150.028.87620531
CGCCAAT32050.028.0653333
GCCAATA32300.027.8479534
TGCTTGA36150.025.268458
GCTTGAA38050.024.5585259
GCGGCCT86700.024.2234611
CCTGCGT87250.024.10858265
GCGTTAT88250.023.87559168
CGGCCTT88350.023.7310512
TCCTGGC88900.023.62135327
Produced by FastQC (version 0.11.2)