FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_10_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_10_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8192238
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC246960.30145608562641857TruSeq Adapter, Index 3 (100% over 50bp)
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG146420.17873015896266686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106420.12990345251199978No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT93350.1139493261792443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33250.053.26048346
ATGCCGT34600.051.3840847
GCCGTCT35300.050.46489349
CGTATGC36000.049.1937844
TGCCGTC36100.049.1523648
GTATGCC36050.049.1255545
CCGTCTT41350.043.33598350
TCTCGTA41450.042.8100441
CATCTCG46300.038.40120739
ATCTCGT46700.038.22218340
TAGGCAT49200.036.06614735
CTCGTAT52400.034.53197542
CTGCTTG55300.033.22618557
TCGTATG55900.032.6829243
GCATCTC57100.031.25994938
TGCTTGA59350.030.13328458
GCTTGAA60200.029.8820459
TGGGGGG7850.029.4443241
CACTTAG65850.027.21316331
TTAGGCA67050.027.03915834