FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_chip_pchx_0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_chip_pchx_0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8861624
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG231680.26144192080368117No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC185770.20963426116928455TruSeq Adapter, Index 1 (100% over 50bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT150590.16993499159973388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25550.056.02446746
GCCGTCT26150.054.4719649
TGCCGTC27050.052.7886748
ATGCCGT27100.052.5618347
GTATGCC27600.051.737345
CGTATGC27600.051.61049744
CCGTCTT33300.042.8816150
TCTCGTA34500.040.67995541
CACGATC37800.037.40608636
ATCTCGT37900.037.30760240
TCACGAT38350.037.0521535
CTGCTTG49700.029.43524757
CTCGTAT51050.029.27435142
TGCTTGA49700.029.15357258
GCTTGAA50550.028.52472359
TCGTATG54350.027.94749643
TGGGGGG6350.027.577221
TTCTGCT63100.023.01833555
CCTGCGT73450.022.77672665
GCGGCCT75900.022.3199411