FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_input_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_input_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8633183
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT114900.13309112062144401TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18600.045.53499248
CGTATGC19900.042.91232746
ATGCCGT20100.042.48533649
GTATGCC20900.041.36123347
GCCGTCT20900.040.85934451
TGCCGTC21700.039.35301250
TCTCGTA29200.029.12569643
CCGTCTT30750.027.99869752
TCACGAG31550.026.7348730
CTCGTAT33650.025.79404844
ATCTCGT33700.025.65192442
TCGTATG35750.024.76836645
GTCACGA37250.023.39529229
ACGAGTG37250.023.11382332
AGTCACG37950.022.87153428
TATCTCG38600.022.2142541
GCACACG38750.022.13133611
ACGTCTG39000.022.07602315
CGAGTGG39050.022.04839533
GAGTGGA38600.022.03354834
Produced by FastQC (version 0.11.2)