FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_input_180_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_input_180_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8746801
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16950.039.43753448
ATGCCGT17350.038.3265949
GTATGCC17550.037.69018647
CGTATGC17650.037.47685646
TGCCGTC18050.037.03456550
GCCGTCT18800.035.3709651
CCGTCTT27250.025.04489352
TCTCGTA28250.023.9104343
ATCTCGT30600.022.41729542
ACGTCTG32450.021.03165615
ACACGTC35400.019.4784213
CTCGTAT35650.019.33983444
GCACACG35600.019.07490311
TCGTATG36450.019.01138545
ACATTCC36100.018.71203432
CGTCTGA37250.018.41538816
TATCTCG36800.018.35515641
CACACGT37000.018.35262712
GCTTGAA41150.016.58482261
TGCTTGA41400.016.31560160
Produced by FastQC (version 0.11.2)