FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_input_180_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_input_180_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10379613
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT115980.1117382700106449TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20500.043.70488448
CGTATGC21750.041.67584246
ATGCCGT21850.041.16515449
TGCCGTC22200.040.5167450
GTATGCC22600.039.79828647
GCCGTCT22900.039.12540451
CACACTG31600.028.24318931
CCGTCTT34800.026.4504452
TCTCGTA35000.025.69931243
GTCACAC36300.024.97166429
ATCTCGT41450.022.46030442
CTCGTAT42100.021.7806844
TCGTATG43700.021.70401645
TATCTCG43950.020.8641641
ACACTGA43500.020.75816532
ACTGATA45550.020.13137834
ACGTCTG45350.020.06561715
GCACACG45900.019.82727611
CTGATAT47500.019.3782435
CTGCTTG49750.018.71252659
Produced by FastQC (version 0.11.2)