FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_input_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_input_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10588333
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT137270.1296426925749313TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23900.044.5164948
TGCCGTC24550.043.19548850
ATGCCGT24550.043.05272749
CGTATGC25300.041.77665746
GCCGTCT25950.041.269751
GTATGCC25800.041.1024947
CCGTACG26950.039.60961533
CGTACGT27850.038.204134
ACCGTAC31850.033.4059432
GTACGTA33600.031.77006535
CACCGTA33950.031.23650631
TACGTAA35650.030.04106536
GTCACCG37150.028.54587629
CCGTCTT38450.027.94404852
AGTCACC38600.027.56396928
TCTCGTA39400.027.00416843
TCACCGT41500.025.7223930
ATCTCGT44950.024.29295742
CTCGTAT45700.023.66429944
TCGTATG45500.023.6144845
Produced by FastQC (version 0.11.2)