Basic Statistics
Measure | Value |
---|---|
Filename | HK72VBGXX_n01_nlp7_4tu_5.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5317510 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT | 52623 | 0.9896173208889123 | TruSeq Adapter, Index 16 (97% over 40bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 16539 | 0.31102903426603806 | No Hit |
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG | 13816 | 0.2598208560021514 | No Hit |
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT | 11719 | 0.22038510505857065 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 6225 | 0.0 | 59.876003 | 48 |
CGTATGC | 6260 | 0.0 | 59.54179 | 46 |
GTATGCC | 6260 | 0.0 | 59.485325 | 47 |
GCCGTCT | 6265 | 0.0 | 59.38311 | 51 |
ATGCCGT | 6305 | 0.0 | 59.11683 | 49 |
TGCCGTC | 6310 | 0.0 | 58.95961 | 50 |
TCTCGTA | 6540 | 0.0 | 56.67258 | 43 |
CCGTCTT | 6715 | 0.0 | 55.50784 | 52 |
ATCTCGT | 6870 | 0.0 | 53.644653 | 42 |
GTCCCGA | 6925 | 0.0 | 52.86432 | 36 |
CGTCCCG | 7055 | 0.0 | 52.08864 | 35 |
CCCGTCC | 7155 | 0.0 | 51.458946 | 33 |
CCGTCCC | 7250 | 0.0 | 50.73638 | 34 |
TGCTTGA | 7540 | 0.0 | 49.43437 | 60 |
CTGCTTG | 7550 | 0.0 | 49.41571 | 59 |
CTCGTAT | 7620 | 0.0 | 48.7785 | 44 |
GCTTGAA | 7655 | 0.0 | 48.737442 | 61 |
TCGTATG | 7735 | 0.0 | 48.279522 | 45 |
CACCCGT | 7910 | 0.0 | 46.72468 | 31 |
ACCCGTC | 7935 | 0.0 | 46.532925 | 32 |