FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5317510
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT526230.9896173208889123TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165390.31102903426603806No Hit
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG138160.2598208560021514No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT117190.22038510505857065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG62250.059.87600348
CGTATGC62600.059.5417946
GTATGCC62600.059.48532547
GCCGTCT62650.059.3831151
ATGCCGT63050.059.1168349
TGCCGTC63100.058.9596150
TCTCGTA65400.056.6725843
CCGTCTT67150.055.5078452
ATCTCGT68700.053.64465342
GTCCCGA69250.052.8643236
CGTCCCG70550.052.0886435
CCCGTCC71550.051.45894633
CCGTCCC72500.050.7363834
TGCTTGA75400.049.4343760
CTGCTTG75500.049.4157159
CTCGTAT76200.048.778544
GCTTGAA76550.048.73744261
TCGTATG77350.048.27952245
CACCCGT79100.046.7246831
ACCCGTC79350.046.53292532