FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_180_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_180_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6444453
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1026181.5923461618852677TruSeq Adapter, Index 19 (97% over 40bp)
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG170990.2653289580977625No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT136170.2112979953457648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93680.14536532425637988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC117400.063.34800347
GCCGTCT117850.063.1382651
TATGCCG117950.063.02392248
CGTATGC118150.062.9458846
ATGCCGT118500.062.7609449
TGCCGTC119100.062.4462250
CCGTCTT122050.061.05156352
TCTCGTA121650.060.7632143
ATCTCGT124650.059.1604142
GCTTGAA132300.056.3983161
TGCTTGA132600.056.32349860
CTGCTTG132850.056.00675659
AAACGAT135200.054.8546237
CTCGTAT136800.054.21220444
TCGTATG139150.053.57332245
CGTGAAA139350.053.2967533
TTCTGCT142350.052.7115857
GTGAAAC141600.052.35181434
CACGTGA142250.052.25941531
GAAACGA143600.051.64585536