FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_180_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_180_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487690
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT785291.21043083131284TruSeq Adapter, Index 18 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174770.2693871008016721No Hit
CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG74680.11511030890810135No Hit
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTT66180.10200857315932173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG92400.061.2095448
CGTATGC92350.061.1668946
GCCGTCT93650.060.42991351
ATGCCGT94150.060.14616449
GTATGCC94000.060.12997447
TGCCGTC95300.059.42037250
CCGTCTT101000.056.0669452
TCTCGTA101800.055.4892543
TCCGCAC103050.054.71343235
CATCTCG104750.053.82630541
CGTCCGC105250.053.70443733
CTCGTAT105400.053.62718244
CCGCACA105900.053.30706836
TCGTATG106600.053.08916545
GTCCGCA107100.052.67873434
CTGCTTG108250.052.31107359
ATCTCGT108300.052.28813642
ACGTCCG108450.052.15207332
CGCACAT108550.052.005737
GCACATC109300.051.58480538