FastQCFastQC Report
Wed 13 Apr 2016
HK72VBGXX_n01_nlp7_4tu_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK72VBGXX_n01_nlp7_4tu_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7896460
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1884582.3866137484391743TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132920.16832859281247547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG210950.063.17828448
GCCGTCT212200.062.8387151
CGTATGC212350.062.74527446
ATGCCGT212650.062.65635749
GTATGCC213150.062.54261447
TGCCGTC214200.062.1874150
CTCGTAT221500.059.94828444
CCGTCTT222650.059.87367652
TCTCGTA221900.059.82444843
TCGTATG224400.059.46988345
CTGCTTG228500.058.4943459
ATCTCGT229450.057.91695442
AATCTCG234350.056.6611741
TGCTTGA243200.054.9583660
GCTTGAA243600.054.8681261
CTTCTGC249800.053.56267556
TTCTGCT250750.053.3876657
TCTGCTT254650.052.62499658
ATGTCAG258800.051.5799134
TGTCAGA259900.051.2798335