FastQCFastQC Report
Mon 26 Oct 2015
HK5NLBGXX_combined_n02_l3b_rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NLBGXX_combined_n02_l3b_rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111287440
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC19399091.7431517878387714No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG13138321.180575274262756No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG8918420.8013860324219876No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG7228820.6495629695498432No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT6841560.6147647928643161No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC4153180.37319395611939676No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC4119560.37017295033473674No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG4083440.36692730105032517No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG3826770.3438636022178244No Hit
CTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATTT3766810.33847575251978124No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG3699200.332400493712498No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC3442020.3092909676060479No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT3401940.3056894830180297No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA3083170.27704563965169837No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC2888880.25958724542500033No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG2762040.24818973282160142No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG2752450.24732800035655417No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT2706990.24324308295707045No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA2650060.23812750118072623No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2615950.23506246527011496No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG2583160.23211604112737252No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA2547840.22894227776288142No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT2444450.21965192118715282No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA2359950.2120589708955476No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA2299940.2066666283275094No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC2285420.20536189888095188No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC2283490.20518847409914362No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG2274770.2044049175720099No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA2267630.2037633357367193No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG2262830.20333202021719615No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT2240340.20131112729343043No Hit
GTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAA2224040.19984645167504975No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT2213400.1988903689401068No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT2102200.18889822607115414No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC2019100.1814310761394098No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC2003890.18006434508692085No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG1943520.17463965385491842No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA1912310.171835204404019No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1904710.17115228816477404No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA1879700.1689049545932587No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1869640.1680009891502581No Hit
CTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAA1789510.16080071569621873No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA1785910.16047722905657638No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG1778420.15980419713132046No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA1760310.15817687961911964No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT1755220.1577195054536253No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT1725660.155063320712562No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA1699490.1527117525571619No Hit
CTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGCTCCTAGGCTAATA1696120.1524089331194967No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC1695210.1523271628855871No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1683220.15124977266077824No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT1678040.15078431132929287No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC1677330.1507205125753634No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT1670680.15012296086602406No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG1639000.14727627843717134No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT1585070.1424302688605291No Hit
CTCAGTCGTGATTACCCGCTGAATTTAAGCATATAACTAAGCGGAGGAAA1581750.14213194229285891No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT1581730.14213014514486091No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT1575810.14159818933744905No Hit
GCTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATT1573510.14139151731767755No Hit
CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC1565600.14068074528446337No Hit
CTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCTATTTCATTATTC1560730.1402431397469472No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC1553350.13957999213568037No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA1548210.139118125100191No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1546160.13893391743039465No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1542680.1386212136787404No Hit
CTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTG1533560.13780171419164644No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA1532030.1376642323697984No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1484470.13339061443052333No Hit
CTTCCGTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA1482750.1332360597026942No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1472780.13234018142568468No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG1464550.13160065502450233No Hit
CTGAATACTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGT1446530.12998142467829252No Hit
CTCTTTTCTGGTTAAGGAACGGACTCCCTGGAATCGGTTCAGCCGGAGAT1446270.12995806175431837No Hit
TTCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA1435460.1289867032613923No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT1427700.12828940983816323No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA1422100.12778620839871957No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC1401740.12595671173674228No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG1391860.1250689206257238No Hit
CTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATC1388100.12473105680209735No Hit
CGGAAAGTTTGGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAG1381960.12417933236670733No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1345850.12093458165629471No Hit
GGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTTCAA1325800.11913294078828662No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA1320720.11867646519679129No Hit
CTCAAAGGCTAAGCCATGCATGTCTAAGTTCAAATAAACCTATAATGGTG1316620.11830804985719862No Hit
CTCAGGTATAGTTCACCATCTTTCGGGTCCCAGCATATACGCTCTACCTC1316330.11828199121122743No Hit
CTCAATGCGGGCTAATGCCTGAATACTCGTGCATGGAATAATGAAATAGG1314530.11812024789140627No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1311170.11781832702774005No Hit
CTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGGAAG1307740.11751011614608081No Hit
CTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTTCAAATAAACCTATAA1299880.11680383698286169No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG1291790.11607689061766538No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC1282770.1152663768705615No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG1279590.11498063033887741No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA1277030.11475059539513176No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG1269970.11411620215183312No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1266510.11380529554817687No Hit
CTGCTTACCAAAAATGGCCCACTTGGAGCACACATTCAATGTCTATGCTT1243460.1117340824804668No Hit
CTAGCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAA1240790.11149416322273206No Hit
CTTTTACTCTACGAGAGGTTTCCGTCCTCTCTGAGCTAGCCTTAGGACAC1237660.111212909561043No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC1229060.11044013592189739No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA1221810.10978866977261764No Hit
CTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAAAGAGGGTGGTAAACCTC1197880.10763838219299501No Hit
CATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTGCA1196010.10747034885518077No Hit
CTTTCATCGATTCTTTACTTATTCGGTAAAACGAGAATTGAAATATTATA1184700.1064540616623044No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA1183900.10638217574238387No Hit
CGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGCCCAACGCTGA1183010.10630220265647229No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC1181910.10620335951658157No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT1178410.10588885861692929No Hit
CTCGTTTAAAATTCGGAGCTTTGGTGCATGTATTAGCTCTAGAATTGCCA1176220.10569207091114684No Hit
CTCGTTATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCG1175220.1056022135112462No Hit
CTCTCATAGATCCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACC1151090.10343395445164341No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA1143450.10274744391640242No Hit
CTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGC1142920.10269981949445509No Hit
CTCGTAGTTGGATCTGCGTCTCAGGACCTGGTCCATCCATTGGATGAGAA1136640.10213551502307897No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT1131860.10170599665155385No Hit
CTTCAACCTATTCTCAAACTTTCAATGAGTATGACGTCTTGCTTGCTTAA1117740.10043721016495663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTT60900.057.6130941
CAACCAG2487550.057.3621833
GCGTCTG2495850.056.9331569
ACCTGGC915850.056.3560834
CCAGACG2551150.056.299576
CAGACGT2587800.055.444577
CACCTGG937250.055.1377373
ACGTACC2596600.055.1288610
CATCGGG2600650.055.01787616
AGTCGTG212350.055.0109254
CGTACCA2612600.054.8394811
GTACCAT2616700.054.6631312
ATGCGTC2610300.054.4474467
TGGCTCA1755900.054.367213
CCCAATG2629050.054.30732329
AATGCGT2617050.054.29862666
CTAGCAT357600.054.1546971
TCAACCC2634200.054.1365625
GCAATGT2638450.053.97855838
CTCAACC2640200.053.9309231
Produced by FastQC (version 0.11.2)