FastQCFastQC Report
Mon 26 Oct 2015
HK5NLBGXX_combined_n02_l3a_rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NLBGXX_combined_n02_l3a_rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146753469
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC28573851.94706470618422No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG20339291.3859495205527306No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG9417950.641753143157386No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT8354150.5692642263877251No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC6762330.46079524021336765No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG6549170.4462702002635454No Hit
CTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTG5122100.3490275245214135No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC4690240.31959994076869147No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG4069770.2773201906389007No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGC3984490.27150908439513616No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA3916640.26688568431728177No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA3898960.2656809427789404No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG3877190.2641975025476229No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA3522670.2400399816102473No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT3212690.21891748262523186No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG3181520.21679351239049754No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC3139570.21393497689652569No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA3110600.21196091793918684No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG3041050.20722167732879965No Hit
CCTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATT3026130.20620500630209976No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA3003740.20467931834715264No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC2933970.19992508660902591No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC2898170.197485621276864No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG2837780.19337055671235953No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC2736820.18649099190970403No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT2722560.18551929426622277No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA2688310.1831854482431349No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA2630730.17926186126475824No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG2529760.17238161504720545No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT2485150.1693418231905646No Hit
CTCAGAATGAGGAGGTAGTGACGAAAAATAACGAGACCGTTCTCTTTGAG2468790.1682270284186604No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT2420370.16492761748616655No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT2372310.16165273748997375No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA2308040.15727328394533557No Hit
CTGAATTTAAGCATATAACTAAGCGGAGGAAAAGAAACTAACGAGGATTT2290010.15604469288559034No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2204070.1501886132586072No Hit
CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC2203490.15014909119456657No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA2201110.14998691444902063No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA2174500.14817366940743323No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG2165830.1475828826915158No Hit
TTCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA2110810.14383373792683565No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC2085890.14213565200288383No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCTCATAGATCCTC2083390.14196529827857085No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC2057710.14021542482242788No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT2045660.1393943198712393No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC2045550.1393868243073695No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG2013440.13719880107229354No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC2005980.13669046555894362No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1993350.13582983854371442No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1979460.13488335325143147No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1978310.13480499053824752No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1963100.13376855847952734No Hit
CATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCGAATTGCA1944620.13250930374940573No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC1915720.13054001469634766No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG1913120.13036284682306215No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT1890000.12878741558061568No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC1873240.12764536421282144No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA1865660.12712885172070448No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG1857790.1265925781965672No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC1849570.1260324551510261No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA1809740.12331837961527166No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1785720.12168162103207249No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA1785240.1216489131170044No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC1750850.119305527285355No Hit
CTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGGAAG1724240.1174922822437676No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT1705440.11621122223693396No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT1683430.11471142804808246No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGC1668110.1136675004254925No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG1661170.11319459848679965No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC1642440.11191830838424678No Hit
CTCGTTTAAAATTCGGAGCTTTGGTGCATGTATTAGCTCTAGAATTGCCA1636730.11152922047791593No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG1628700.11098204431542263No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA1607910.10956538274403585No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT1603070.10923557793376593No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA1593800.10860390632401336No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA1593460.10858073821750681No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA1589930.10834019875877686No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT1578180.10753953625450585No Hit
CTGAATACTCGTGCATGGAATAATGAAATAGGATCTCGGTTCTATTTTGT1553320.10584553881993755No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG1511910.10302379973041727No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC1511870.10302107407082826No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC1508580.10279688856963237No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT1471880.10029609589671779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT390050.060.12331
CTTGTTA932550.054.7695961
CAACCAG3887500.054.5795333
TCCAATC436450.054.1589552
GCGTCTG3946000.053.85074669
TGGCTCA2776750.053.3680573
CCAGACG4054500.053.0324486
ACCTGGC1211350.052.7627074
CAGACGT4124200.052.0864377
CTCAACC4123850.051.6230771
ACGTACC4168050.051.46350510
CATCGGG4171650.051.32385616
CGTACCA4185550.051.2959711
CACCTGG1250300.051.1334883
ATGCGTC4161100.051.12656867
GTACCAT4195800.051.08397312
AATGCGT4177900.050.9098166
CCCAATG4248400.050.25576429
TCAACCC4256950.050.14679325
GCAATGT4260800.049.96367338
Produced by FastQC (version 0.11.2)