FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614182
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25070.408185195919125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG559.559523E-519.09365530
AGCGCCG550.002100410616.36332510
GTATAGA1750.016.2880571
TAGTACT653.4381167E-416.1509084
GCTCTAA809.7284465E-515.0021591
TCAGGGG750.001018396613.9974543
TAATACT1557.754352E-913.5459234
TAAGGCT902.652877E-413.3309084
CTATATA800.001653086213.1268891
ATTAGAC800.001657146413.1226133
TAGACAG800.001657146413.1226135
ATTGTAC1151.09302255E-513.0411063
TATCCTG1855.0567905E-1012.97061355
ATCCTGG1302.753035E-612.6920666
TATTATG1551.1293014E-712.5804062
TATAGAG1456.8797453E-712.4135572
GTATTAT1702.8105205E-812.3547191
AGACTCC850.002610243912.3527066
AGACTAG850.002610243912.3527067
CATAGTA850.002610243912.3527062
Produced by FastQC (version 0.11.2)