FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4627604
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100340.21682927061174637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG13300.09.8110295
GTATAAT18050.09.6419291
GTATATA25500.09.2960831
TTCTATA13800.09.1298982
GTATAAG11800.09.0273321
CTACACA6650.09.0216364
GTATAGA12550.08.9660391
TATAATA34650.08.9605132
ACTATGC9100.08.9002888
CTAATAC9750.08.7685273
CCTACAC4301.8189894E-128.720233
TACACAG8100.08.7028175
TAATACT19100.08.6378634
TACACCG5450.08.531245
TATTATA30150.08.5069642
TATACAG16950.08.494715
CTTACAC4601.8189894E-128.477583
TTATACT14700.08.4685254
GTATTAA12550.08.3683031
GTATTAG9700.08.3522821
Produced by FastQC (version 0.11.2)