FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4592656
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64390.14020209656460228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10850.011.20011
CTAGGAC3451.8189894E-129.9985773
GTATTAA13700.09.52721
ACTGTGC5750.09.3900718
GAGCGCA3603.6379788E-119.1657629
ATACGGT10950.09.0399086
GTATATA35200.09.0356591
TATACGG10750.08.7895435
ACGGTGT9250.08.7556068
GTATTAT22000.08.7287861
ACCGTGC2752.4489236E-78.7261268
CCCCTAT3801.07320375E-108.6857181
GTCTAGA5700.08.6857171
TACGGTG9450.08.5703037
CTAATAC11200.08.57020953
GTATAGA11950.08.5370461
TATACTG16350.08.530995
CTAGGGT3757.6397555E-108.3988964
CTGTGCG2157.532093E-58.3712669
TATACCG8450.08.1646085
Produced by FastQC (version 0.11.2)