FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472097
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21030.14285743398702666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCC650.006392108713.8445398
CTAGTAC2206.2937033E-1011.5895573
GGACAGT2259.131327E-1011.3320116
GGCTCGG1408.175158E-510.7144921
GCTCGGA1408.18362E-510.71342
AGTGTCC1851.1222583E-610.539318
CTAATAC3900.010.3834053
CATATAC5900.010.1683043
GTATTAA4750.010.1054571
GTCCTAA1952.2006752E-610.0001931
GTCTTAT2557.185008E-910.0001931
ACTATGC2557.19956E-99.9988348
TCTATAC3305.456968E-129.9988343
TATACAG5300.09.9045055
CTACACC2651.3513272E-89.6215194
TAAGACT2054.1737603E-69.51108554
GGACCAT1603.1499207E-49.3739076
GTATAGC2251.3457502E-69.3335131
TAGACTG1450.00134013749.3092595
CTATGCA2601.0338408E-79.2296929
Produced by FastQC (version 0.11.2)