FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6453376
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97490.1510682160779102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG4250.09.53070451
GTATTAG16450.09.4845691
TATACTG25050.08.9806795
TAGGTAG8900.08.9312345
GTCTTAG7400.08.9201291
GTATATA53550.08.7406821
TATACAG27600.08.422645
GTGTAAG10250.08.3425951
TAGACTG7000.08.1416275
CTAATAC16250.08.121853
TCATATA32600.08.0971872
TTCTATA26500.08.0933682
TCTACAC12350.08.0149823
CCTATAC11100.07.97176843
GTATATG29600.07.957161
TATTATA65000.07.9375082
CTTACAC9100.07.91089253
CTAGTAC9900.07.8775983
TATACCG12200.07.74473145
TATAATA65900.07.7380692
Produced by FastQC (version 0.11.2)