FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4800042
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77370.16118608962171582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA13450.010.0387961
TATACTG17800.010.0269935
TAATACT19150.010.0250134
CGCGCAC1250.00414626959.59944910
TAGGTAG5850.09.4861425
TATACAG21950.09.4294925
TATACCG9000.09.3323135
TACACTG8050.09.3157515
ACTATGC9750.09.2298568
GTATAAG13400.09.0686291
GTATTAG12100.09.0510481
GGTAGTC5100.08.8226577
GTATATA38300.08.6175791
CTAATAC12200.08.6056163
GTCCTAC3501.8480932E-98.5728131
CTACACA7900.08.5433694
GTATTAA17800.08.512611
GTGTAGG4800.08.4388631
CCGTGCA3203.189234E-88.4367539
TTATACT18500.08.4315094
Produced by FastQC (version 0.11.2)