FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069123
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73870.24068764920793334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG3100.012.5795795
CGGCCGG1354.215155E-612.22198315
CGCCGCG1459.338011E-611.38001424
TGCGACG4850.010.516830
CGACGGG1150.002077911710.4338998
GTATATA23100.010.1975961
GCGCGGC1501.645874E-49.99947912
ACCGCGC1356.9676084E-49.9991528
TATACGG5600.09.9098745
GTCTTAT5000.09.9027111
TCTATAC8200.09.8772133
GTGCGAC5350.09.53392229
GCGGCCG2054.174066E-69.5120114
TACACCG3001.0550139E-99.4991955
GCCGCGT1757.4213225E-59.42946325
GTATTAG7400.09.3268781
GGTGCGA5000.09.30118328
GTATAGA8550.09.3007911
TCCTAGG1952.3905777E-59.2305882
TGCGCGG1952.3914972E-59.23028811
Produced by FastQC (version 0.11.2)