FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511249
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39560.7737912445794515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATA350.00299878521.4348621
ACATAGG400.006513018618.7463326
GGGTATT550.002102241916.36043511
TACATAG859.2003465E-615.8792475
TTAGCCT750.001018092313.9972624
AGTACTG750.001018092313.9972625
TGTCTCT650.006367258713.85021930
GTACTAC650.006367258713.8502191
GTGTATA800.001650564813.1288521
CAGTACC800.00165665713.1224334
TATATCG1056.7478744E-512.8546285
CCTTATA1201.6923099E-512.4975552
GTATAAG1006.4223097E-412.0035231
AAGACAG1202.3435193E-411.2477995
GTATAAA2351.8480932E-910.8542491
TATAAGG1253.4205083E-410.7978872
AATACTG1951.8903665E-710.7671245
GTCTTAT1408.128486E-510.7174311
ACTGTTC1551.9522717E-510.6430788
ATACTGC1150.002074735510.4327416
Produced by FastQC (version 0.11.2)