FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365800
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88190.1643557344664356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC1356.9702783E-49.9990196
GTCTATA6350.09.9224131
GTATATA16900.09.7644481
TATACTG14150.09.53975
GTATTAA27350.09.489221
CTAAGAC6700.09.1780333
GTATAAG10150.09.0158261
TATACCG6500.08.9991165
CTTATAC9350.08.8224983
GTATTAT24300.08.58124351
TCTATAC7050.08.50964453
TATATAC17350.08.3851883
TTATACT17950.08.3556484
CAGTGCG2701.7574221E-68.3325929
TAGACAG5450.08.256075
TAGTACC6450.08.138664
GTATAAC10550.08.10520651
TAATACT24500.08.01954654
CTGTGCA6250.07.9192979
CTAGACA3808.638381E-97.89388854
Produced by FastQC (version 0.11.2)