FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n02_ntr_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415802
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37340.26373744351258155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCG500.001110492517.99976721
TAGACTG1551.1337943E-712.5800375
ACAGCGC900.00400931411.6661048
TATACAG4400.011.590355
TTATACT5400.011.3883394
GTATTAG4000.011.2526371
GTCTTAT1755.458551E-711.1454691
TAGACAG2853.6379788E-1211.0520985
ATTATAC5700.011.0520983
CTAATAC3500.010.7137683
TAGGTAG1551.9558172E-510.6446475
TGCTCGT1000.00878190810.49949410
TATTATA8750.010.4566372
GTATTAA5050.010.3984761
TGTGCGA1451.16741736E-410.34432810
GGTCTAT1756.59358E-610.2881251
ACAATCG1356.9603435E-49.9995178
GTATAGA4100.09.8803631
GTCTAAG1552.2816047E-49.67968751
TCCTATA2651.3495082E-89.6221772
Produced by FastQC (version 0.11.2)