FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614182
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTAC7470.12162518601977915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGA350.003005146721.4278721
TCTCTAC2000.018.7539730
CTCTCTA1850.017.84161629
CTCTTAT1650.016.3631041
ACCTCGC550.00210174416.361778
CCTTAAA653.4342092E-416.153321
TCTTATA2150.015.3483372
TAGTCCT901.5796564E-514.9995124
AGGACTC706.03268E-414.9995115
CCTAACC706.03268E-414.9995113
CTAACCC706.03268E-414.9995114
CTTAAGG952.6320055E-514.2100623
TATTATA952.6320055E-514.2100622
GTCTAGA650.00638296213.8457021
ATACGAC650.00638296213.8457023
ACCCTGC650.006386406313.8445768
GTACTAG902.6495272E-413.33291
GTATATG902.6495272E-413.33291
TAGGACC902.6495272E-413.33294
ATCATAC800.00165528513.1245733
Produced by FastQC (version 0.11.2)