FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4627604
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG4050.010.7412135
GTATTAG10150.010.3460651
AACCCCG5950.09.83236528
TATACTG16750.09.7616235
CTTACAC4950.09.697293
CCGTGCG2209.937521E-79.5450499
GTATAGA12800.09.3761211
TAATACT18850.09.2311744
TAGACAG6550.09.1607085
TCCGAGC5600.09.10685413
GTATTAA14500.09.1045381
TAGGTAG5300.09.0570025
TTATACT15250.09.0495784
CCGAGCC5150.09.02883914
AGGGGGG3851.4551915E-118.9614325
TAGACTG3358.112693E-108.9556185
CCACGAG5900.08.89811920
TACACTG7600.08.8819695
TATTATA29750.08.8743392
TACACAG8300.08.8558115
Produced by FastQC (version 0.11.2)