FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4592656
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG4300.011.1632175
TAGGTAG5800.010.3452225
GTATTAG10050.010.0007081
AGCGCAC3401.0913936E-119.705090510
GTATTAA13300.09.6999341
ACCGTGT2651.3544195E-89.6218568
CTAATAC12900.09.5352473
TAGGGGG2105.6689805E-69.2860694
CGGACTC4700.09.25617630
CCTATAC9100.09.2311213
GAGCGCA2651.3878889E-79.0558649
GTATAGA12150.08.8895181
GTACTAG8650.08.8445561
CTTATAC13250.08.8305243
TCTTATA15800.08.829452
GTATATA33600.08.7506191
CATATAC18350.08.7469273
GACGGAC3952.5465852E-118.73460528
TATACAG19000.08.6845415
TTATACT15600.08.6541764
Produced by FastQC (version 0.11.2)