FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472097
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA800.001656848813.1249416
CTTAGAC1753.072273E-912.8570853
GTGTAAG1702.8245267E-812.3541441
ACCGTGC850.002614283512.3520468
CTGGACG1006.45111E-411.9999464
TGGGGGG1303.8453778E-511.5384095
TACGGTG2659.094947E-1211.3199357
ACTGTCC1602.2641198E-611.2491858
CCCCTAT1253.4194894E-410.8010521
TAATACT6500.010.6153364
ACGGTGT2556.4028427E-1010.5874688
GGACGAG1000.00878376310.49923911
GTACCGA1304.9162854E-410.3845686
GTATTAA4300.010.1172651
AGGGGGG1200.00295481189.9999545
GTATAGA4500.09.6676081
TAGGGGG1409.7131723E-49.6428144
GTATTAG3451.4551915E-119.5661491
ATACGGT3001.0495569E-99.4999576
CTAAGAC1901.7564544E-59.4736413
Produced by FastQC (version 0.11.2)