FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6453376
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG5250.013.4293355
AGGGGGG4350.010.3454155
CCGAGCC5250.09.7138614
GTATTAG16950.09.4705191
TATACTG26900.09.2570315
GTATATA56400.09.0439781
CCGTGCG1500.00182619678.9993959
TATACCG11250.08.9338415
TCCGAGC4900.08.87695513
CTTAGAC8250.08.7277013
TAGACAG11050.08.6882765
ACCGTGC3654.474714E-108.6296248
TCTTATA21500.08.5818172
TAGGGGG4054.5474735E-118.5190024
TAGGTAG9900.08.4853315
CTAATAC17150.08.3969143
TATACAG30550.08.2983445
TAATACT30800.08.2796924
TATAATA68700.08.210012
GTCTAGA6950.08.2027311
Produced by FastQC (version 0.11.2)