FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4800042
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG3100.013.064574
AGGGGGG3450.013.0435325
TATACTG17050.010.8211595
GTATTAG11600.010.7339221
TAGACAG7950.09.8113615
CTAAGAC5750.09.6522143
GTCCTAG3451.4551915E-119.5662541
TAGGTAG7100.09.5070825
TAGACTG5950.09.327775
CCTGGAC2601.0345866E-79.2308083
CCGGGTG1300.00572749749.2304239
CCTATAC8650.09.190793
GTATAGA13700.08.9790751
CCTGCAC4350.08.9655543
CTAATAC13900.08.9568723
TTATACT19300.08.93786054
GCCCGAC1350.00780838968.88855611
GTATATA37300.08.8481441
CTAGGAC3451.4133548E-98.6956883
TACACCG5650.08.495615
Produced by FastQC (version 0.11.2)