FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069123
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2351.2732926E-1112.128455
GACCGCG1252.5786465E-511.9992187
GTATTAG7050.011.2786171
CTAATAC7550.011.1265523
TCCGAGC3650.011.09516715
TGCGACG4950.010.909801530
GTGCGAC4950.010.909801529
CCGAGCC3750.010.79929516
TACAGGC6600.010.6811227
TCTTATA10300.010.6317612
ATACAGG10250.010.0982196
CACGCAG5800.010.0855516
TATACAG15050.010.06710155
CTTATAC9100.010.05563
GGTGCGA5250.010.00065228
TAGGTAG3900.010.0006515
CGGGGCT1200.00295665779.99983823
ACCGCGC1200.00295785549.9993498
ACAGGCA6700.09.8501048
TCTAGAC3051.364242E-109.8367063
Produced by FastQC (version 0.11.2)