FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511249
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGA13810.27012277774626453No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAG10200.19951139268732065No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTAGAGG5380.10523247967233187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT2400.021.882851
TAGAGGA2800.017.67799630
GTAGAGG2950.015.762197529
TTAGTCT750.001014584214.0036544
CTAAGAC750.001015335114.0022833
CCATAGT650.00636863613.8497684
TCTTATA3900.013.8484112
TTTATAC2351.2732926E-1112.1296373
GTATAGT1151.569862E-411.7433431
TATAGGC900.003997009711.6685693
CTTTATA1806.214759E-811.6685692
TATACTA1551.5326423E-611.6159335
TATTATA2751.8189894E-1211.4564132
TTATACA6200.011.3739354
CGTAGAG4500.011.33296430
CGAGCCC4700.011.16875617
GCCCACG4850.011.13365720
GAAGTGT950.0059772711.0555166
TATACGT950.005981625511.0544352
CTGTCTC950.005990343611.05227229
Produced by FastQC (version 0.11.2)