FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365800
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3400.014.1184415
TGGGGGG4300.013.9542735
CTCCGAG4200.010.7137912
CGAGCCC4350.010.68906115
TAGACTG3357.2759576E-129.85135
CCGAGCC4750.09.78902114
TAGGGGG4150.09.7595854
AGCCCAC5200.09.51870117
GCCCACG5850.09.48682918
GTATATA15550.09.3578751
CACGAGA5950.09.32764721
TATACTG13550.09.1887085
GTATTAA23850.09.1204351
GAGTGCG2505.8067417E-78.9996687
ACGAGAC5850.08.97427822
CCCACGA6050.08.9252919
GTATAAG10800.08.8898031
CGAGACA6250.08.88000223
CCACGAG6650.08.79691320
GTATTAG15400.08.7671361
Produced by FastQC (version 0.11.2)