FastQCFastQC Report
Mon 9 Nov 2015
HK5NHBGXX_combined_n01_ntr_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415802
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGT1056.753178E-512.8568858
CACCGTG850.002613170512.3526947
GTATTAA4400.012.2733491
ATTATAC6650.011.95507053
GGACTGG900.00400635311.6672576
TATTATA10550.011.6589462
GTATTAG4200.011.429151
AGGGGGG1059.7091054E-411.429155
TAATACT5650.011.1506144
TAGTCAG1355.6505203E-511.1108899
TCTTATA4350.011.0346522
TAAGACT2207.743438E-910.9092574
ATAATAC7200.010.6251623
TTATAGT4400.010.5683434
GACTGGG1000.00878006810.499797
GTCTTAG1451.1662484E-410.3453511
CTAATAC4100.010.244063
TAGACTG2352.1618689E-810.2132845
GTATATA9550.010.0528651
GTCTATC1501.6427485E-410.0005061
Produced by FastQC (version 0.11.2)