FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728661
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT165510.606561240110076No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC128710.4716965573957337No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC116130.42559335879392857No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC99640.36516078765372467No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC99520.36472101151443875No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG92350.3384443871921063No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG85350.31279077906709557No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC82410.30201626365459106No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT77960.2857078984894056No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT75060.2750799751233297No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG74720.27383394272868633No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT73160.26811685291796966No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG60150.22043778981705678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58140.21307153948401797No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT55900.20486238488401456No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC55370.20292004026883514No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT54300.19899870302686923No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG54190.19859557489919047No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC54170.19852227887597615No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG51520.18881055580007922No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT50370.18459603446525605No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT49820.18258039382686234No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG49590.1817374895598977No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA49390.18100452932775452No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC49230.18041816114203998No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC46880.17180587841435782No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG44740.16396320393042596No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC44740.16396320393042596No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG43990.16121460305988908No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA43780.16044499481613875No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT42270.15491114506345785No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC41530.15219919220452816No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC40900.1498903674732772No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC38380.1406550685482733No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC37910.13893261200273688No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT37520.1375033395500577No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG37300.13669708329470023No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT37150.13614736312059283No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT36010.1319694897973768No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT35490.13006379319380457No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT35000.12826804062505381No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC34680.12709530425362475No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG34440.12621575197505297No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA34390.1260325119170172No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA34210.12537284770808832No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT34170.12522625566165968No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC32850.1203887181295148No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA32330.11848302152594258No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA31590.11577106866701287No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC31380.11500146042326255No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC30760.11272928370361873No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG30730.11261933966879725No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA30680.11243609961076147No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA30430.11151989932058251No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC30240.1108235871000465No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT29950.10976079476343892No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT29930.1096874987402246No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG29700.10884459447325996No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC29640.108624706403617No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC29290.10734202599736649No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA28600.10481331319647255No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT28590.10477666518486539No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG28440.10422694501075803No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC27990.1025777844884359No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA27740.10166158419825694No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA27530.10089197595450662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG13200.025.6879061
ACCCGTG13100.025.6460043
CCGTGCT13750.024.2146035
CCCGTGC13900.024.061194
ACCTAGA1650.022.7301628
CCTAGAT1700.022.06162629
ACCGTAT28600.021.6055957
CCACCGT28700.021.5299195
CCGTATG28700.021.4780528
TACCTAG1750.021.43129327
TGTTAGG15200.021.41719429
GTGTTAG15100.021.36033228
GTTAGGC15550.021.2253430
ACTCGGT5700.021.0502913
TATGCGC16350.021.00760811
TGTGTTA15300.020.98305927
CATGTGT15800.020.9829125
CGTATGC29450.020.8801489
GTATGCG29800.020.78589810
CGACTTG15800.020.69731718
Produced by FastQC (version 0.11.2)