FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748072
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT294870.7867244812799754No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC207110.5525774318102747No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC154850.4131457453325336No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC152770.40759622547272306No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG131830.3517275014994376No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG121030.3229126868427287No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC108280.2888951973174475No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT107360.28644060199483895No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG105100.2804108352240832No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG104770.27953038255401713No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC104680.27929025909854455No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT98030.26154780377751546No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT96850.2583995184724306No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG91830.24500596573384928No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT83280.22219423746395478No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG83200.22198079439242363No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC82610.2204066517398812No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA82200.21931275599828393No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC78510.20946769432390838No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG78060.20826707704654554No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT76750.20477194675022253No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC76160.20319780409768007No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT75920.20255747488308654No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG75690.20194382605243444No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74640.19914238573858772No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT72480.193379422807246No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC72020.19215212514594168No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT68760.18345431998104625No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC67570.18027935429202002No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG67030.17883861355918457No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG65590.17499663827162337No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT61500.164084361239592No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT60310.16090939555056571No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT58700.15661385373600079No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA58510.15610692644111426No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC58050.15487962877980999No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG56890.15178470424260793No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT56440.15058408696524506No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC53900.14380726944413022No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA52540.1401787372281002No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC52410.13983189223686204No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC50920.1358565150295939No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC50700.13526954658288315No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC50320.13425569199311008No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC49400.13180109667050152No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT49370.13172105551867733No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA48830.1302803147858419No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG48760.13009355209825213No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA47880.12774567831140918No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA47610.12702530794499145No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG47200.12593141220339416No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT46040.1228364876661921No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT45480.12134238616547388No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA44900.11979492389687285No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC43440.11589958784142887No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT42710.11395191981370689No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC42550.11352503367064452No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC42520.11344499251882034No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA42320.1129113848399924No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC42220.11264458100057842No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA41030.10946961531155218No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG40880.10906940955243123No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA40870.10904272916848981No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA40790.10882928609695865No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT40670.10850912148966188No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG39750.10605452616705335No Hit
CCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTAT39100.10432030121086254No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC39080.10426694044297975No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC38960.10394677583568299No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC38500.10271947817437872No Hit
GTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG37650.10045164553935998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG18850.026.1842821
CCCGTGC19750.025.2885074
CCGTGCT19700.025.1242895
ACCCGTG20050.024.6109073
ACTCGGT8250.023.6339043
TGTTAGG21650.022.17510229
GTGTTAG21800.022.15986628
GTTAGGC22150.021.94488330
TGTGTTA21750.021.9351927
TACCTAG2200.021.82231527
CTTGCAT22000.021.7497721
ACTTGCA22450.021.58134820
CTCGGTC8950.021.4502814
TGCATGT22400.021.42862923
CATGTGT22200.021.42183325
GACTTGC22800.021.38192219
CGACTTG22700.021.34395418
CTAGATA2250.021.33680530
ATGTGTT22450.021.18356526
TTGCATG22400.021.16048822
Produced by FastQC (version 0.11.2)