FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662099
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT206200.7745767531560621No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC102220.38398271439191406No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC90160.33868011670490095No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG79130.29724664634936565No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG75100.28210821611067055No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG60740.22816581952812423No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT60070.22564900854551237No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT59580.2238083557373336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58470.2196387136616632No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC57310.21528124987087258No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA56180.21103647910915407No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG55430.20821915338234978No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT54900.20622824320207478No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG54710.20551452068461765No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC53840.20224642284152466No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT53060.1993164040856482No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG52670.19785139470771No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG46800.17580112535258832No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT45590.1712558398466774No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC44970.16892685057918583No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT43630.16389322861396213No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC42870.1610383385441338No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC42400.15927281442200308No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG42320.15897229967781062No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC42120.15822101281732948No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG41700.1566433104103191No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC41470.15577933052076576No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT39000.14650093779382362No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT38240.14364604772399522No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA36820.1383119110145791No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG35570.13361636813657193No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT35400.13297777430516297No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC34820.1307990424097676No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC34510.12963454777602185No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC34310.1288832609155407No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA34040.12786902365389116No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT33700.1265918359910732No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA33580.12614106387478452No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT33060.12418771803753355No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG32890.12354912420612456No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT31590.11866575961299712No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC31400.11795203709554002No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA31160.11705049286296265No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC30160.11329405856055691No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT29910.11235494998495547No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT29880.11224225695588332No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC29770.11182904918261868No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG29340.11021378243258421No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT28810.10822287225230917No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC28710.10784722882206861No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG28270.10619439772901007No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC28180.10585631864179355No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC28140.10570606126969734No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC27700.10405323017663881No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC27250.10236283474055624No Hit
GTCGTTACTCATGTCAGCATTCGCACTTCTGATACC27210.10221257736846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTG6050.023.3035243
ACTCGGT5300.022.0733283
GTACCCG6550.021.9867271
CTCGGTC5300.021.5073454
TAGCTTA350.003008875521.426498
CCCGTGC6900.020.867544
TATGCGC14300.020.76838511
GCCATAC35600.020.4372481
CATACCC35300.020.3521333
CCACCGT21500.020.2305955
CCGTATG21500.020.1608338
TACCCTT35200.020.1116855
CCATACC36000.020.0397222
ACCGTAT21800.019.9521927
CATCCAC21950.019.6108532
ATCCACC22000.019.4299323
CACCGTA22400.019.35086
CCGTGCT7400.019.2548895
CGTATGC22650.019.1372159
GTATGCG23050.018.8705410
Produced by FastQC (version 0.11.2)