FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296722
Sequences flagged as poor quality0
Sequence length36
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT13540.4563193831262933No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12420.4185736143595689No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC11900.4010487931464468No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC11190.377120671874684No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT8990.3029771975114754No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC8000.2696126340480315No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT7090.23894419692506794No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG6570.22141937571194584No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT6330.2133309966905049No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC5560.1873807806633819No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT5560.1873807806633819No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG5090.17154103841306004No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG5080.1712040226205No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG4910.16547475414697932No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT4850.16345265939161907No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC4670.15738637512553838No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA4540.15300516982225787No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC4300.14491679080081693No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC4160.14019856970497638No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG4150.13986155391241634No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG4140.1395245381198563No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG3980.1341322854388957No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC3950.13312123806121556No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC3840.1294140643430551No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC3840.1294140643430551No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT3800.12806600117281497No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT3740.12604390641745472No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG3720.12536987483233464No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC3720.12536987483233464No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT3610.12166270111417421No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA3550.11964060635881396No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC3510.11829254318857381No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC3480.11728149581089369No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC3390.11424835367785334No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC3280.11054117995969293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCTT456.731352E-726.6743130
ATAGGGA456.7511974E-726.665322
TAGGGAC456.7511974E-726.665323
AGGGACC456.7511974E-726.665324
GTGTTAG359.943069E-525.71732128
TGTGTTA359.943069E-525.71732127
CTGCCAA359.9544704E-525.7129861
GCCATTA300.001223568325.00294924
GATAGGG501.6913218E-623.9987871
TGTTAGG452.0295185E-523.34002129
GTTAGGC402.4567574E-422.50644930
CATGTGT402.459563E-422.50265525
CACCGTA3600.022.4988636
CCGTATG3550.022.3932348
ACCGTAT3550.022.3932347
TATGCGC1750.022.28458811
CCACCGT3800.021.7094275
ATTAGGT350.003001840321.4274883
ATCCACC3850.021.4274863
TCCACCG3850.021.0378974
Produced by FastQC (version 0.11.2)