FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541689
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT28570.527424407732112No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC17240.31826380081559713No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC16100.2972185146827792No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT13600.25106657140905575No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC12930.23869785061169788No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG12310.22725216867981443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11630.21469884010936166No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT11080.2045454125891425No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG10870.2006686493541497No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC10670.19697649389225186No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG10100.18645385082584287No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG9010.16633160355849944No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG8580.158393469315419No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT8500.15691660713065986No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG8220.15174758948400283No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC7990.14750161070282025No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC7790.1438094552409224No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC7480.13808661427498067No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG7410.1367943598633164No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC7350.13568671322474704No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT7340.13550210545165214No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT7260.134025243266893No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC7070.13051769557809No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA6970.12867161784714107No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC6920.12774857898166658No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG6880.12701014788928702No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG6750.1246102468390534No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC6590.12165652246953511No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG6550.12091809137715552No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA6280.11593368150359339No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT6060.11187231049550574No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG6050.11168770272241083No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC5940.10965701721836699No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT5800.10707250839503848No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC5510.10171888297528656No Hit
GTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAG5490.10134966742909676No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG5470.10098045188290698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT200.00514705229.9974144
GTATGAA200.00514705229.9974141
TCCAATA702.3628672E-923.5693992
TTTAATT652.7823262E-823.0749362
TTAATTC706.098344E-821.4267253
TAATTCG706.098344E-821.4267254
CCAATAT657.092294E-720.7674433
ATTACTA455.454414E-420.00381729
AGGACGT455.454414E-420.00381728
TACCCTT5450.018.9891915
CATACCC5450.018.9891913
TCTCTTA400.0064989918.75357830
GTTTAAT802.4928522E-718.7483841
ATACCGT400.006509392518.7483846
CCATACC5600.018.480552
GCTATCT1950.018.46506127
GGAGAAC1950.018.46165318
TTCCCCA651.6445105E-518.459959
ATTCCCC651.6458187E-518.4582448
GCCATAC5550.018.3767951
Produced by FastQC (version 0.11.2)