FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478079
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT41900.8764241893076249No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC19550.4089282315265887No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC18960.39658717492297296No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG14250.29806789254495597No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT13820.2890735631558801No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG12930.27045739302500216No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC12820.268156518065006No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG12320.25769799551956896No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT11580.2422193821523221No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT10540.22046565525781306No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG10470.21900146210145185No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC10040.21000713271237598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10010.20937962135964974No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC9830.20561455324329242No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC9360.19578354205058163No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG9210.19264598528695048No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA8280.17319313335243755No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG8260.1727747924506201No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG8050.16838221298153652No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT7830.1637804630615442No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC7470.15625032682882956No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC7420.15520447457428582No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG7270.1520669178106547No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT6640.13888917940340403No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC6350.13282323632705054No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG6310.1319865545234156No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC6110.12780314550524077No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT6100.127593975054332No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC6000.12550227054524463No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA5740.12006383882161736No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT5700.1192271570179824No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC5590.11692628205798625No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT5440.11378872529435513No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG5430.11357955484344638No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC5380.11253370258890268No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC5370.11232453213799393No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA5280.11044199807981527No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA5250.10981448672708904No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC5130.10730444131618415No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT5070.1060494186107317No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA4860.10165683914164812No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT4800.1004018164361957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG801.6370905E-1124.3848611
GCCATAC6950.022.6710381
CATACCC7050.022.552883
CCATACC7100.022.3940542
GTAGGAT553.877698E-621.8178793
TACCCTT7250.021.7238355
ATTCGTG2350.021.70182422
TAAACTG350.00300385921.4282725
CATTCGT2400.021.24970421
TCGTGCA2350.021.06353624
ATACCCT7750.020.9029354
ATCGTTA2400.020.62471211
TTCGTGC2500.020.39971523
ACCCTTG7900.020.12636
GTCTCTT455.460662E-419.99972230
TAGGATA608.235698E-619.9997224
TAGGAAG608.235698E-619.9997225
TGTAGGA852.2107997E-819.4114932
AGGATAG701.4421548E-619.2854445
CCATTCG2650.019.24501420
Produced by FastQC (version 0.11.2)