FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289291
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44381.5340954264045545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA452.0205882E-523.3529821
TATTAGA608.230178E-619.9960822
TATACCG601.8599207E-417.4995965
TAGACCA653.4261085E-416.1534735
TATACTG706.01857E-414.9996545
TATACTC809.7139695E-514.9996545
TATATTA809.7283024E-514.9970612
GTATAGA851.6151853E-414.1295371
AGAGTAG650.006371813.8458355
CTGTGTA650.006371813.8458359
TACACAT1302.738887E-612.6920155
AAGAGTG954.1714223E-412.6312885
TATTACA954.177513E-412.6291052
ATATACT1201.6833248E-512.4997114
TATTATA1354.1862913E-612.2198282
ATACACA1252.5586454E-511.9997234
TATATTG1501.0303665E-611.99972255
TTGTAGA1006.433882E-411.9976492
TATAGAA1006.433882E-411.9976492
TATAACA900.004000556711.6643812
Produced by FastQC (version 0.11.2)