##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n02_ntr_8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8 Sequences flagged as poor quality 0 Sequence length 36 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 32.0 0.0 0.0 0.0 0.0 0.0 4 32.0 0.0 0.0 0.0 0.0 0.0 5 32.0 0.0 0.0 0.0 0.0 0.0 6 36.0 0.0 0.0 0.0 0.0 0.0 7 35.5 0.0 0.0 0.0 0.0 0.0 8 35.5 0.0 0.0 0.0 0.0 0.0 9 33.25 0.0 0.0 0.0 0.0 0.0 10 33.25 0.0 0.0 0.0 0.0 0.0 11 33.25 0.0 0.0 0.0 0.0 0.0 12 36.0 0.0 0.0 0.0 0.0 0.0 13 36.0 0.0 0.0 0.0 0.0 0.0 14 36.0 0.0 0.0 0.0 0.0 0.0 15 35.5 0.0 0.0 0.0 0.0 0.0 16 36.0 0.0 0.0 0.0 0.0 0.0 17 35.5 0.0 0.0 0.0 0.0 0.0 18 33.25 0.0 0.0 0.0 0.0 0.0 19 36.0 0.0 0.0 0.0 0.0 0.0 20 35.5 0.0 0.0 0.0 0.0 0.0 21 33.25 0.0 0.0 0.0 0.0 0.0 22 36.0 0.0 0.0 0.0 0.0 0.0 23 35.5 0.0 0.0 0.0 0.0 0.0 24 35.5 0.0 0.0 0.0 0.0 0.0 25 36.0 0.0 0.0 0.0 0.0 0.0 26 36.0 0.0 0.0 0.0 0.0 0.0 27 35.5 0.0 0.0 0.0 0.0 0.0 28 36.0 0.0 0.0 0.0 0.0 0.0 29 36.0 0.0 0.0 0.0 0.0 0.0 30 35.5 0.0 0.0 0.0 0.0 0.0 31 35.5 0.0 0.0 0.0 0.0 0.0 32 36.0 0.0 0.0 0.0 0.0 0.0 33 35.5 0.0 0.0 0.0 0.0 0.0 34 33.25 0.0 0.0 0.0 0.0 0.0 35 36.0 0.0 0.0 0.0 0.0 0.0 36 35.5 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 33 2.0 34 0.0 35 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.5 25.0 12.5 0.0 2 25.0 0.0 37.5 37.5 3 12.5 50.0 25.0 12.5 4 0.0 25.0 50.0 25.0 5 0.0 25.0 37.5 37.5 6 25.0 62.5 12.5 0.0 7 0.0 12.5 50.0 37.5 8 25.0 62.5 12.5 0.0 9 37.5 12.5 37.5 12.5 10 12.5 37.5 12.5 37.5 11 12.5 37.5 37.5 12.5 12 37.5 25.0 12.5 25.0 13 25.0 25.0 37.5 12.5 14 25.0 25.0 37.5 12.5 15 25.0 12.5 0.0 62.5 16 12.5 50.0 0.0 37.5 17 25.0 12.5 12.5 50.0 18 12.5 37.5 25.0 25.0 19 0.0 25.0 25.0 50.0 20 0.0 12.5 62.5 25.0 21 12.5 25.0 37.5 25.0 22 0.0 25.0 50.0 25.0 23 0.0 12.5 37.5 50.0 24 12.5 37.5 25.0 25.0 25 37.5 12.5 12.5 37.5 26 0.0 25.0 37.5 37.5 27 37.5 25.0 25.0 12.5 28 37.5 25.0 25.0 12.5 29 0.0 0.0 62.5 37.5 30 37.5 50.0 0.0 12.5 31 12.5 50.0 12.5 25.0 32 0.0 12.5 37.5 50.0 33 50.0 12.5 37.5 0.0 34 25.0 37.5 12.5 25.0 35 25.0 12.5 37.5 25.0 36 12.5 37.5 50.0 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.5 36 1.0 37 1.0 38 1.0 39 1.0 40 1.0 41 1.0 42 1.0 43 1.5 44 2.0 45 2.0 46 1.5 47 1.0 48 1.0 49 1.5 50 2.0 51 1.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 8.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATATATGGGATGGTATTTTCGATGTTCGTTTGT 1 12.5 No Hit GCACTAATGCTACTACCACTGCCAGCATCAACGACA 1 12.5 No Hit ACATTACAACACGGCAACACTAATCACGCAACGCTA 1 12.5 No Hit GTATAATAGAAGGCCAGACCATCCTCTCTAACTACT 1 12.5 No Hit TTGTAGTGGATTTACCCTCTCCCAGTGGTGTTGGTG 1 12.5 No Hit GCACCACATTTCTTCAGGCTTTTCCTGATGCTGGTT 1 12.5 No Hit ATCACGCACACGAGGACTTTCTTAGATATGCAAAAA 1 12.5 No Hit GGTTCTTGTCAATACCCCAAAATTCCAGCAACTCGT 1 12.5 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE