FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n02_ntr_89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n02_ntr_89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823165
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62360.22088684154131974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGG5600.018.48564726
CCCGGAG5900.017.54321117
CCGGCCC6500.017.53712513
GCCCGGG6700.017.24207325
CGGCCCG6500.016.8448714
CCCGGCC6500.016.8448712
CCGGGGC6150.016.10089927
GGGCCCG7500.015.79991523
GCCCGGA6650.015.563827516
AGCCCGG5900.015.50619510
CGGAGGG6750.015.33406619
GAGCCCG6000.015.2477599
GCCCGGC7600.014.80071811
GGAGCCC6150.014.6319968
CCGGAGG7600.014.40858418
AGGAGCC6400.014.2947737
GAGGGCC7800.014.03863621
GGAGGGC7900.013.48165720
AGGGCCC8400.013.39302322
AAGGAGC7750.012.1917556
Produced by FastQC (version 0.11.2)